- Wild-type minimal inhibitory concentration distributions in bacteria of animal origin in Argentina.
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- Effect of peritoneal fluid pH on outcome of aminoglycoside treatment of intraabdominal infections
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resistente; CIM, concentración inhibitoria mínima. Tabla 1. Métodos para el estudio de susceptibilidad antimicrobiana. Es necesario señalar. La terapia por encima de la concentración inhibitoria mínima (CIM). Curvas de muerte bacteriana y efectos persistentes de los antibacterianos. concentración de sal, pH y temperatura afectan .. Concentraciones mínimas inhibitorias de aceites esenciales probados “in .. ages/files_pdf/brasil/greentinphalihang.tk
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Se realizó la prueba de E- test® para establecer la concentración inhibitoria mínima a tetraciclina, ciprofloxacina, amoxicilina, metronidazol, azitromicina y. PDF | On Jan 1, , D Domingo and others published Plantas con acción antimicrobiana. Content be subject to copyright. Download full-text PDF. Contra cada . concentraciones mínimas inhibitorias (CMI) altas ( mg/l ) en. PDF | Se estima que un tercio de la población mundial esta infectada por Mycobacterium Determinación de la Concentración Mínima Inhibitoria de Aceites Esenciales frente a Cepas de Mycobacterium tuberculosis. Data (PDF Download.
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Wild-type minimal inhibitory concentration distributions in bacteria of animal origin in Argentina.
Ho, J. Agents Chemother. Sabath, L. In Action of Antibiotics in Patients, L. Sabath, editor. Bern, Hans Huber, , pp. Hays, R.
Of the Enterococcus spp. This is the first report in Argentina showing data of epidemiological cutoff values in animal bacteria. All rights reserved.
Todos los derechos reservados. De los Enterococcus spp.
Antimicrobial resistance is a global public and animal health concern that is influenced by both human and non-human antimicrobial usage. The human, animal and plant sectors have a shared responsibility to prevent or minimise antimicrobial resistance selection pressures on both human and non-human pathogens Epidemiologists need to be aware of emerging changes in bacterial susceptibility, which may indicate emerging resistance, and allow for appropriate control measures to be considered3.
In an attempt to overcome the problems of differences in interpretative criteria based on clinical or epidemiological data, the European Committee on Antimicrobial Susceptibility Testing EUCAST has decided to define separate dividing points for the detection of bacteria with resistance mechanisms and the monitoring of resistance development using wild-type cutoff values WCV or epidemiological cutoff values ECOFF or ECV and the guidance of therapy via clinical breakpoints9.
Data are inserted into a database and each distribution is screened for acceptance, and then made freely available5.
Spectra were recorded in the — cm-1 wavenumber range by using a resolution of 4 cm-1 and sample scans. Samples were analyzed in uniplicates. Briefly, melanin extracts were dissolved in a 0. The mobile phase consisted in methanol:acetic acid , that was applied in an isocratic elution during min flow rate: 0.
Melanin filtered solutions were diluted in acetonitrile and homogenized in an ultrasound bath 37 KHz for 5 min. The mass spectra were processed using flexAnalysis 3. Statistics The statistical analyses were performed by using the software GraphPad Prism version 5. Results Antifungal Activity of Melanin Minimal inhibitory concentration values of the different melanin extracts against C.
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According, to the origin species of the melanin, MIC values average and range obtained against C. Moreover, MIC values observed against C. Bars represent mean and standard error of the mean SEM of three replicates. Additionally, we tested the antimicrobial activity of the melanin extracts against eight clinical strains of C. The human melanin showed a significantly higher antifungal activity against both C.
Minimal inhibitory concentrations values of human melanin, fungal melanin extracted from C. Our results revealed that the human melanin displays as ellipsoidal deposits in accordance with melanosomal structures, similarly to a previous study Liu et al.
On the other hand, the structure of the synthetic melanin as well as the melanin obtained from C.
Effect of peritoneal fluid pH on outcome of aminoglycoside treatment of intraabdominal infections
The main elements detected on the surface of melanin were carbon C and oxygen O which are present at equivalent percentages on each melanin extract analyzed Figure 4. An interesting difference between melanins was the presence of sodium, which was only detected in the synthetic melanin.
Melanin particle sizes were estimated from SEM images. Red dots: carbon, green dot: oxygen, and blue dots: nitrogen. The spectra of melanin obtained from the extracts from different organisms are presented in Figure 5.
A wide band in zone of cm-1can be observed, corresponding to links vibration of the functional groups -OH and -NH2. In Table 2 , the transmission patterns of fungal melanin extracts showed a similar spectroscopic curve, suggesting the presence of similar functional groups.
However, differences in the intensity of signals can be observed in the and cm-1 zone in human and fungal melanins compared to the synthetic one. This is related to C-C links of the aromatic ring of the molecule. Melanin characterization by fourier transform infrared spectroscopy FTIR for A human melanin upper blue profile and synthetic melanin extracts lower red profile , B C. Fourier transform infrared spectroscopy FTIR peaks for different melanins.
The human melanin also exposed a minor peak around 13 min Figure 6B.
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The chromatograms of fungal melanin were similar to synthetic and human melanin similar to synthetic and human melanin, in terms of the main chromatographic peak, as shown in Figures 6A,B. However, all cryptococcal melanin chromatograms exposed another peak nearby to 14 min with a variable intensity, suggesting the presence of more than one compound in the fungal extracts Figures 6B,C. It was observed an abundant ion at Da, with high relative intensity in all extracts and also, a less intense ion at Da.
The mass spectrum of the fungal extracts suggested the presence of two or three different compounds, as well as in the HPLC chromatograms. No signals were observed in the range of 2,—10, Da, therefore, those mass spectra were not included in this article. Mass spectra obtained from A synthetic and human melanin, B C.
Discussion In this study, the antifungal activity of melanins from different biological origins was tested. To date, this is the first study to correlate the variation of the antifungal activity with the physicochemical characteristics of melanins. It has been previously described that the efficiency of the melanin extraction from different biological origins is low due to factors associated with the molecule and the source Ito and Wakamatsu, In the present work, a sample of human hair from a healthy volunteer was used as a source of human melanin.
In this case, the melanin extraction efficiency reached to 3. In the current study, human and synthetic melanin showed an important inhibitory activity against all tested Cryptococcus strains Figure 2 and Table 1. In addition, this antifungal activity against the clinical isolates, obtained from patients, was higher than the reference strains Figure 3. In previous work Tapia et al.
Therefore, these results support the idea that human melanin can represent a mechanism of antimicrobial defense against fungi. Similar to our preceding results obtained against Candida species Fuentes et al. Although that Candida sp and Cryptococcus sp are two distinct microorganisms, this data suggests that Candida species would be more sensitive to synthetic melanin than Cryptococcus species.
Similarly, to synthetic melanin, the MICs obtained with the human melanin were slightly lower in C. The antifungal activity of melanin extracted from Cryptococcus strains was significantly lower than those extracted from human and synthetic melanins.
Human melanin showed an activity 14 times higher than the cryptococcal melanins. In addition to strain dependent effect, these results show that the antifungal activity of melanin is also dependent on the originating organism. In the current study, we tested the antifungal activity of fungal melanin extracts against the fungal species where they had been extracted from, showing that the activity was lower against strains of their own species.
This should be subject to further studies. The physicochemical characterization of extracts revealed that melanin was the main component in all the analyzed extracts.
Although nitrogen is also present in the melanin structure, the proportion of N atoms in the melanin molecule is relatively low with respect to the other majority elements.In addition, eight clinical C. The results showed significant differences in antioxidant ities Choi et al.
Si el pus se colecciona hacia el esternocleidomastoideo, se forma lo que se conoce como absceso de Bezold.
The chromatograms of fungal melanin were similar to synthetic and human melanin similar to synthetic and human melanin, in terms of the main chromatographic peak, as shown in Figures 6A,B. Fu, K.
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